Native Chromatin Immunoprecipitation from Brain Tissue Using Magnetic Beads
نویسندگان
چکیده
منابع مشابه
Magnetic Beads Make Chromatin Immunoprecipitation Faster and Easier
1 Active Motif’s ChIP-ITTM Express Kits use protein G-coated magnetic beads instead of the traditionally used agarose beads to make chromatin immunoprecipitation (ChIP) simpler and faster than ever before. The use of magnetic beads has made it possible to simplify and streamline the ChIP protocol, eliminate a number of timeand labor-intensive steps and dramatically reduce background. The result...
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Native chromatin immunoprecipitation refers to a method that allows for identification and quantification of DNA that is associated with specific chromatin proteins without altering the structure of these proteins. The method has been used with great success in the past and has some advantages over the more widely used cross-linking chromatin immunoprecipitation. We describe here a protocol tha...
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In cells and tissues, the histone proteins that constitute the nucleosomes can present multiple post-translational modifications (Luger & Richmond, 1998), such as lysine acetylation, lysine and arginine methylation, serine phosphorylation, and lysine ubiquitination. On their own, or in combination, these covalent modifications on the core histones are thought to play essential roles in chromati...
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Epigenetics remains a rapidly developing field that studies how the chromatin state contributes to differential gene expression in distinct cell types at different developmental stages. Epigenetic regulation contributes to a broad spectrum of biological processes, including cellular differentiation during embryonic development and homeostasis in adulthood. A critical strategy in epigenetic stud...
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Occupied Regions of Genomes from Affinity-purified Naturally Isolated Chromatin (ORGANIC) is a high-resolution method that can be used to quantitatively map protein-DNA interactions with high specificity and sensitivity. This method uses micrococcal nuclease (MNase) digestion of chromatin and low-salt solubilization to preserve protein-DNA complexes, followed by immunoprecipitation and paired-e...
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ژورنال
عنوان ژورنال: Medicinal Chemistry
سال: 2014
ISSN: 2161-0444
DOI: 10.4172/2161-0444.1000223